Accumulation of heavy metals in the environment is of major concern as they are not biodegradable and therefore causes serious health problem to living organisms. In recent years, biosorption of heavy metals by microbes and agro-waste materials have gained increasing attention over the conventional methods. A total of one hundred and twenty fungi were isolated from soil samples collected form heavy metal contaminated sites. Two species of Aspergillus exhibited resistance to 1500mM of lead (Pb) on agar medium. A biosorbent was designed by growing these fungal organisms on luffa sponge (an agro-waste material) under shaken condition for 72 hours at 300 C. The percentage of lead adsorption by the designed biosorbent was determined initially by gravimetry method and further confirmed by Atomic Adsorption Spectrophotometry (AAS) and Inductively Coupled Plasma- Optical Emission Spectrometry (ICP-OES) showing 35%, 56%, 63% respectively with A niger, whereas for A terreus it showed 32%, 44%, 59%. The Fourier Transform Infrared Spectroscopy (FTIR) analysis revealed that the functional groups OH- , =CH+ present on our designed biosorbents are facilitating the biosorption. The textural properties of the designed biosorbent was morphologically analyzed by Scanning Electron Microscopy (SEM).The practical applicability of the designed biosorbent for heavy metal removal from heavy metal contaminated environment is discussed.
Full conference title:
- ASM microbe 1st (2016)