Bioassay for itraconazole

Itraconazole standards are placed in duplicate in a pre-arranged order and distributed throughout the plate. Patient samples of serum or plasma are placed in duplicate in other wells. A standard curve is drawn using the measured diameters of the standards and the patients' samples then determined. The bioassay measures both itraconazole and the major bioactive metabolite of itraconazole - hydroxitraconazole.