Bacterial and fungal growth in thermos bottles: a microbiological survey

R.Watschinger, H.Hutter, G.Fluch, C.Jebelean, L.Binder, M.Haditsch, H.Mittermayer, AT.Linz


Introduction: Thermos bottles are used widely in private households as well as in the hospital setting. The most frequently used thermos bottles consist of five parts: a screw-on top, an O-ring, an isolating bottle, an outer bottle and a screw-on bottom. Materials and methods: 56 thermos bottles from three different hospitals and 8 bottles from private households were investigated for bacterial and fungal growth. The bottles were visually inspected for cleanliness and two swabs were taken from each bottle, one from the O-ring at the top of the bottle and one from the inside bottom of the bottle after taking it apart. Results: All of the swabs, both from visibly clean as well as from visibly contaminated bottles gave rise to bacterial ggrowth; nearly all of them yielded fungal growth. We isolated not only Gram-positive bacteria such as Micrococcus spp., coagulase-negative staphylococci and Bacillus spp., but also Gram-negative bacteria such as E. coli, Klebsiella pneumoniae, Enterobacter aerogenes, Enterobacter agglomerans and other enterobacteriaceae. We also found Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Acinetobacter baumannii, Acinetobacter lwoffi and other nonfermentative Gram-negative rods as well as yeast species such as Candida spp., Rhodotorula spp., and several molds including Aspergillus fumigatus.

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)