In the solid-state culture, Aspergillus oryzae exhibits phenotypes such as the high production of enzymes, conidiophore development, and the production of various kinds of secondary metabolites. Though these characteristics should involve various gene expressions, the only a few regulatory systems have been understood. From the EST database of A. oryzae, we found two genes encoding transcription factors that show high homology to atf1 of Shizosaccharomyces pombe and named atfA and atfB respectively. The atfB gene was upregulated at the late phase of solid-state culture, while atfA gene was constitutively expressed. We tried to identify atfB target genes by microarray analyses. In the atfB-overexpression strain, about 30 genes including catalase gene (catA) were upregulated. Almost all of these genes were upregulated at the late phase of solid-state culture in wild type strain. Furthermore the expression of these genes were downregulated in the atfB-disrupted strain. The disruptant strain grew normaly but its conidia lost several stress tolerances.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)