L-asparaginase is an amidohydrolase that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia. Asparaginases are classified into two categories, type I and type II, the latter being regulated. Aspergillus nidulans has two asparaginase genes, apnA (on chromosome II) and ahrA (on chromosome VIII). The enzyme expressed by the ahrA gene is categorized as a type II asparaginase [Shaffer et al. (1988) Mol. Gen. Genet. 212, 337-341]. Since Cereon Genomics, LLC (subsidiary of Monsanto), had sequenced the entire A.nidulans genome, we supplied them with the sequences of five asparaginases (type II) and received a single matching sequence (contig ANIC1307). From this sequence we prepared PCR primers and will use them with genomic DNA to produce a nucleotide sequence containing one of the L-asparaginase genes. We will clone this sequence into a vector in order both to amplify the DNA for sequencing and express it for enzymatic testing of this possible asparaginase gene (apnA orahrA). Since type II asparaginases are used as a cure for childhood acute lymphoblastic leukemia, this research may have some pharmaceutical significance.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)