The ascomycete Aspergillus nidulans is a model organism to study fungal development. The expression of the brlA gene triggers the formation of the developmental structures, the conidiophores. The expression of brlA is regulated by a number of upstream regulators, including FluG, FlbA-E. In addition to these regulators, we have found that the product of gcnE is necessary for the expression of the brlA gene. GcnE is a homolog of yeast GCN5p, the catalytic subunit of the conserved SAGA/ADA complexes responsible for the majority of lysine acetylation in histone H3 (H3ac) and subsequent transcription-related chromatin remodelling. In A. nidulans, deletion of gcnE results in a severe defect of asexual development. In this study, we compared wild type and the gcnE) mutant by transcriptome analysis and chromatin modification assays. Microarray analysis revealed major effects on the expression of genes involved in primary and secondary metabolism as well as in development. Consistently, the expression of brlA is dramatically reduced and some of the upstream regulators are deregulated. Chromatin immunoprecipitation assays revealed an altered pattern of H3ac in promoters of the conidiation regulators, suggesting that H3 acetylation carried out by GcnE is required for the accurate regulation of conidiation.
Full conference title:
26th Fungal Genetics Conference
- Fungal Genetics Conference 26th (2005)