Various anti-inflammatory and anticancer drugs were tested using a bioassay, with the mitochondrion-dependent sexual structure (ascus) of the yeast Eremothecium ashbyi as indicator. When the anti-inflammatory drugs (some also anticancer) aspirin, ibuprofen, indomethacin, salicylic acid and benzoic acid were tested, similar results were obtained as previously hypothesized i.e. development of the ascus with increased mitochondrion activity was more sensitive to these drugs than the less active asexual growth stage. Here ascospore release nano-mechanics, which are dependent on oxylipin production by β -oxidation, are the most sensitive. Consequently higher concentrations of these drugs are necessary to inhibit asexual growth. The same was found for the anticancer drug Lonidamine, antimalarial drug Artemisinin as well as traditional medicine such as pepperbark. The results suggest that certain antimicrobial, anti-inflammatory and anticancer drugs have anti-mitochondrion activity targeting the yeast sexual phase at low concentrations, This bio-assay may find application in the screening for novel drugs with multiple actions from various sources.
Full conference title:
17th International Society for Human and Animal Mycology
- ISHAM 17th (2009)