Antimicrobial susceptibilities of anaerobic bacteria and comparison of the E-test, ATB ANA and agar dilution susceptibility testing techniques

O. Koru, M. Ozyurt, C. Acikel, H. Aydogan, A. Basustaoglu


A total of 322 strains were isolated from different clinical specimens. These isolates were tested for susceptibility to eight antibiotics (Penicillin G, Amoxicillin/Clavulanic acid, Cefoxitin, Imipenem, Chloramphenicol, Vancomycin, Metronidazole, Clindamycin) by using two different methods which are ATB-ANA strips, PDM Epsilometer test (E-test) strips. The results were compared with those of gold standard agar dilution method which were performed according to the NCCLS criteria. Imipenem was found as the most effective agent in vitro among the agents tested(100%). Susceptibility to penicillin G, amoxicillin/clavulanic acid, cefoxitin, chloramphenicol, vancomycin, metronidazole and clindamycin are 36.7, 83.3, 88.3, 96.6, 30, 85 and 90, respectively. E-test have showed statistically a good correlation (r =0.62,P = 0.001) with the results of agar dilution (total agreement for all antibiotics changing between 90.01 and 98.45%) and a moderate correlation (r =0.45,P = 0.048) with the results of ATB-ANA method (total agreement for all antibiotics changing between 75.46 and 98.76%). However, it was concluded that the routine use of agar dilution procedure might cause some difficulties due to its difficult and nonpractical applicability and requiring experienced personal to interpret.

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)