Antifungal susceptibility and virulence factors of strains Candida spp isolated in Ceara - Brazil

Menezes E.A., Cunha F.A., Neto J.G.L., Amorim L.N., Barbosa A.L., Cavlcante J.E.

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Candida albicans is the Candida species most frequently isolated from patients with candiduria. However, other species with more reduced susceptibility to antifungal agents, such as C. parapsilosis, C. tropicalis, C glabrata, C. krusei, and C. guilliermondii, are teadily increasing their isolation frequency. The pathogenesis of candiduria involves several factors, among which may be counted germ tube and hypha formation, adhesion factors, phenotypic switching, and slime production, as well as the production of different enzymes. The purpose of this study was to evaluate the susceptibility and virulence factors of strains Candida spp isolated of urine in Ceara - Brazil. The strains were streaked onto Potato glucose agar plates (Mumbai, India) at 37C for 24 to 48 h. After this period, the strains were streaked onto chromogenic media agar plates and incubated at 37C for 24 to 48 h. Twelve yeasts isolates have been evaluated (8 C. albicans, 2 C. tropical is, I C. glabrata, I C. parapsilosis). Slime and exoenzymes (proteinase, coagulase, and phospholipase) production tests and determination of their levels were performed. Susceptibility testing was evaluated in medium Mueller- Hinton agar supplemented with glucose and methylene blue was used for disk diffusion testing. Paper disks containing fluconazole, itraconazole and amphotericin B were used. Plates were inverted and incubated for 24 h at 35C. Zones of inhibition were read and interpretation of zone diameters was as described in CLSI M44-A. The following reference strains were also included in the studies: C. albicans ATCC 10231, C albicans ATCC 14053, C. parapsilosis ATCC 22019. In the present study, a higher phospholipase and coagulase activity was also observed for C. albicans. All strains were susceptible to amphotericin B. Candiduria is even more common in the setting of indwelling catheters. Of concern is that candiduria is associated with higher mortality, especially in patients with comorbidities.

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17th International Society for Human and Animal Mycology
    • ISHAM 17th (2009)