Antifungal Effect of Soxhlet Extract of Thymus vulgaris Leaves on Fungal Isolates from Barbering Equipment

L. C. CHIDI-ONUORAH, C. A. OYEKA

Author address: 

NNAMDI AZIKIWE Univ., AWKA, Nigeria

Abstract: 

Background:With the aim of ascertaining the efficacy of some plants known to have antifungal properties on fungal organisms and developing new antimicrobial agents, this study was carried out and involved twenty (20) barbershops.Methods:Two hundred (200) samples were collected from barbering equipment pre and post sterilization. The equipment included electric clippers, brushes, combs and scissors. The work tops were also sampled. Samples were inoculated onto Sabouraud Dextrose Agar (Micromaster Thane, Maharashta, India) incorporated with 0.5mg/ml of chloramphenicol and incubated at room temperature for 5 - 7days. Subcultures were carried out to purify the isolate. Isolates were identified microscopically and macroscopically. Soxhlet and crude extracts of Moringa oleifera seeds and leaves, peels of Citrus sinensis (orange) and C. limon (lemon) and leaves of Thymus vulgaris (thyme) were obtained and the mean zone of inhibition of the isolates to extracts determined at 500mg/ml. The mean minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of the effective extracts were also determined.Results:A total of one hundred (100) fungal isolates belonging to six (6) genera were recovered. These included Trichophyton schoenleinii (20), Microsporum ferrugineum (14), Aspergillus nidulans (8), Scedosporium apiospermum (4), Rhizopus microsporus (6), A. glaucus (6), M. nanum (15), A. niger (12), A. versicolor (10), C. albicans (5). All extracts with the exception of orange peel Soxhlet (OPS), thyme Soxhlet (TS) and thyme crude (TC) extracts were ineffective on the isolates. The MIC and MFC values ranged from 62.5 to 500mg/ml and 250 to >500mg/ml respectively.The antifungal effect of the extracts to the isolates was significantly different (p
2016

abstract No: 

SUNDAY-429

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)