Anti-Inflammatory Effects of Moxifloxacin (MXF) on IL-8, IL-1946; and TNF-945; Secretion and the MAP-Kinase ERK Activation in Human Monocytes Stimulated with Aspergillus fumigatus (AF)

I. SHALIT, D. HALPERIN, S. WERBER, D. HAITE, A. LEVITOV, Y. ROMANO, N. OSHEROV, I. FABIAN

Author address: 

Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.

Abstract: 

Background: We have previously shown that moxifloxacin (MXF) conferred protective anti-inflammatory effects in a murine model of Candida pneumonia in immune suppressed animals (AAC 2002, 46:2442). Further in-vitro studies showed anti-inflammatory effects of MXF in LPS and cytokine stimulated monocytic and epithelial cells (43rd ICAAC). Methods: In the present study, aiming at more clinically relevant conditions for immune suppressed patients, we studied the effect of MXF on monocytic cells stimulated with conidia of a clinical isolate of Aspergillus fumigatus (AF293). Human peripheral blood monocytes (HPBM) and human monocytic cell line (THP-1) were incubated with 2% human serum and stimulated for 6h with AF conidia (1.5x106/ml). Cytokines secretion and MAP-kinase activation were measured with and without the addition of MXF (5-20μg/ml). Results: AF stimulation increased secretion of IL-8, IL-1β , and TNF-α from HPBM by 4.3, 15.8 and 8.7-fold respectively. Addition of MXF (5-20μg/ml) inhibited the three cytokines secretion by 37-50%, 32-76% and 45-60% respectively (p
2004

abstract No: 

B-1695-200

Full conference title: 

44th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 44th