Anti-biofilm Efficacy of Liposomal Amphotericin B and Echinocandin Catheter Lock Solutions against Candida Species

M. Simitsopoulou, D. Kyrpitzi, A. Velegraki, T. J. Walsh, E. Roilides

Author address: 

Aristotle Univ., Thessaloniki, GREECE

Abstract: 

Background: Candida biofilms (BF) form on central vascular catheters and cause refractory candidemia that frequently requires catheter removal. Antifungal lock therapy may be an alternative treatment ofCandida catheter infections. Liposomal amphotericin B (LAMB) as well as anidulafungin (ANID), caspofungin (CAS) and micafungin (MFG) are differentially active against biofilms of non-albicans Candidaspp. (Simitsopoulou et al. AAC 2013). We compared the pharmacodynamic activities of LAMB and 3 echinocandins against resistant Candida spp. BF. Methods: Mature BF of C. lusitaniae (CL; n=6) and C. guillermondii (CG; n=5) blood isolates and C. albicans M61 (CA; control) were produced by shaking 106blastoconidia/mL in RPMI at 37oC for 48h. Biofilms were then incubated with no drug or with ANID, CAS, MFG and LAMB at 4-fold increasing concentrations from 0.007-2048 mg/L for 24h. BF MIC was determined as >50% fungal damage (FD) compared to controls by XTT colorimetric assay in pentaplicate on 3 separate experiments. Data from different Candida species were compared by ANOVA with Bonferroni post test. Results: While ANID and MFG at concentrations ranging from 0.007-2048 mg/L showed reduced activity against BF of CL (FD
2013

abstract No: 

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Full conference title: 

53rd Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC, 53rd (2013)