Antagonistic Activity of Bacteria Against Root Rot Fungal Pathogens of Ginseng

A. Sundararaman1, J. Young2, C. Sung3, S. S. Lee1

Author address: 

1Kyonggi Univ., Suwon, Korea, Republic of, 2Agricultural research and Extension services, Suwon, Korea, Republic of, 3Gyeonggido Agricultural Res. and Extension services, Suwon, Korea, Republic of

Abstract: 

Korean Panax ginseng C.A. Meyer with thousand years of history, has been traditionally known as medicinal plant with mysterious powers in the orient. The efficacy of ginseng has been recognized based on oriental medical science theory and determined that ginseng roots and its extracts revitalize the body. Ginseng crop cultivation is highly vernacular to phytopathogenic fungi such as Rhizoctonia solani, Cylindrocarpon destructans, Collectotrichium and Botrytis cinera. The phytopathogen outbreak leads to replant disease in which the crop cannot be cultivated for consecutive years in the same soil. Our studies focuses on the biocontrol ability of bacterial species against fungal pathogens of ginseng. The bacterial strains were isolated from the rhizosphere of ginseng and in vitro tests were performed to check the efficiency of antagonism. Two effective strains were subjected to 16S rRNA partial gene sequencing and found belonging to the genus Bacillus and Paenibacillus. In vivo analysis was performed in pots under shade at green house conditions. The results of pot experiment showed the efficiency up to 67% reduction in root lesions. Bacterial inoculum was prepared in bulk and the culture filtrate was analyzed for the active metabolites responsible for the antagonistic properties. The culture filtrate analysis showed that the compounds belong to the surfactant group of lipopeptides such as Iturin and Plipastatin. The crude metabolites were purified and analyzed by LC-MS to confirm the mass of the metabolite and identify the molecular structure. Further work focuses on the identification of the gene corresponding to antifungal property.
2016

abstract No: 

SATURDAY-071

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)