Analysis of Growth Characteristics of Filamentous Fungi in Defined and Undefined Nutrient Media.

D.T.A. TE DORSTHORST1,2, M. JANSSEN1, A.J. RIJS1, P.E. VERWEIJ1

Author address: 

1 University Medical Center Nijmegen, Nijmegen, Netherlands, 2 Canisius Wilhelmina Ziekenhuis, Nijmegen, Netherlands

Abstract: 

Background: RPMI 1640 was chosen by the NCCLS as standard medium for in vitro antifungal susceptibility testing, although there was no evidence that this medium adequately supported the growth of molds. The aim of this study was to compare the growth characteristics of molds in various undefined and defined media with RPMI 1640. Methods: The growth of Aspergillus fumigatus, A. flavus, Scedosporium prolificans, Fusarium oxysporum, Rhizopus microsporus and R. oryzae was determined in undefined media (Antibiotic medium no 3, Iso-sentietest broth, Sabouraud liquid medium, Mueller Hinton II broth and Casitone agar) and defined media (Yeast Nitrogen Base, RPMI 1640, Hanks balanced salts, SAAM-F, MEM, NCTC-135 and D-MEM/F12). The molds were inoculated in a microtiter plate and growth curves were constructed by sequential spectrophotometric measurements of turbidity during 72h of incubation. The growth was analyzed by comparing the maximum ODs (ODmax). Results: The growth was better supported by the undefined media than the defined media. Among the defined media SAAM-F supported the growth significantly better than RPMI 1640 (P
2002

abstract No: 

M-1484

Full conference title: 

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    • ICAAC 42nd