Analysis of gene expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinitis: A transcriptomic analysis

Author: 

Vanherberghen M, Bureau F, Peters IR, Day MJ, Clercx C, Peeters D
Vet Microbiol. 2011 Dec 14. [Epub ahead of print]

Abstract: 

<p>Sino-nasal aspergillosis (SNA) and lymphoplasmacytic rhinitis (LPR) are two common causes of nasal discharge in dog. SNA is typically due to an invasion of Aspergillus fumigatus in the surface of nasal mucosa. The etiology of LPR is poorly understood and a possible implication of fungi is suspected. The purpose of the present study was to explore the immunopathogenesis of these diseases by comparing gene expression in the nasal mucosa from dogs affected by SNA or LPR with healthy dogs, using a canine-specific microarray and quantitative real-time reverse transcriptase polymerase chain reaction for confirmation of the findings of the microarray study. Total RNA was isolated from biopsies of nasal mucosa and gene expression was analyzed via hybridation to the Affymetrix GeneChip(à‚®) Canine Genome 2.0 Array. Selected Affimetrix probes sets identifiers were downloaded into the Database for Annotation, Visualization and Integrated Discovery. Genes of interest were chosen after their fold change and their possible implication in immunopathogenesis of SNA or LPR. The results presented here were in concordance with previous studies on SNA and LPR and highlighted new molecules potentially involved in the pathogenesis of SNA. The over-expression of interleukin (IL)-16, natural killer cell group 7 and chemokine ligand 10 might be related to a potential protective Th1 immunity counterbalanced by other molecules such as DNA-binding protein Ikaros.Copyright à‚© 2011 Elsevier B.V. All rights reserved.</p>