Agrobacterium tumefaciens-mediated transformation has been successfully applied to the phytopathogenic fungus Phytophtora palmivora. The transformants were resistance to hygromycin B as the selective trait, governed by Aspergillus nidulans gpd promoter andA. nidulans trpC terminator. The transformants appeared to be stable through mitotic and meiotic cell division. The presence of the hph gene was detected by PCR. Our findings indicate that the technique offers potential as an alternative tool to conventional transformation techniques and should address to the P. palmivora pathogenicity and decision-makes to disease management.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)