Objective: To assess the in vitro antifungal activity of some locally available pharmaceutical preparations used to cure skin diseases. Most commonly used preparations were evaluated for dermatophytic and non-dermatophytic fungi viz Aspergillus niger, Trichophyton rubrum, Candida albicans, Rhizopus microspores and Cryptococcus neoformans. Method: Clinical isolates and collection strains of Candida, Aspergillus, Cryptococcus neoformans Trichophytion rubrum and Rhizopus microsporus were used. One candidal species isolated from interdigital mycosis. Cryptococcus neoformans isolated from patient cerebrospinal fluid was collected aseptically in a sterile bottle by puncturing subarachoniod space between third and fifth lumber vertebrae. Rhizopus microsporus was isolated from the eye of infected patient and Trichophyton rubrum was isolated from skin of patient. Disc diffusion method was used for inhibition zone of different preparations. Results: When compered with different preparations used against skin diseases, Zalim lotion were found to be highly active against Trichophyton rubrum, Candida albicans and Rhizosporus microsporus. The antidermatophytic activities of the Zalim lotion by disc diffusion technique is given in table 1. Zalim lotion exhibited the maximum activity with a halo of 30 mm against Trichophyton rubrum and Candida albicans, followed by Cryptococcus neoformans and Aspergillus niger. Other availabale marked lotions viz Rinco lotion B - Tex, Ring zone, D, K oil,kanji oil, Razal cream, Kanjii oil was inacative against these pathogenic fungal species. None of the creams was found to be protected enough against these fugal strains. Conclusion: The results obtained from the experiment suggest broad spectrum activity of Zalim lotion against dermatophytic and non dermatophytic fungi. However no reports on the antifungal activity of the selected preparations from India have been so for come across the literature .The results of the present study indicated the presence of active antimicrobial agents in Zalim lotion and support the folkare usage of studied preparations.
Full conference title:
- ISHAM 19th (2015)