4. Genome sequence analysis of Chrysosporium lucknowense C1: a filamentous fungus of biotechnological importance.

Hans Visser1, Sandra Hinz1, Martijn Koetsier1, Vivi Joosten1, Scooter Willis2, Bruce Pascal2, and Jan Wery1.

Author address: 

Dyadic Netherlands, Wageningen, Netherlands. 2Scripps Institute, Jupiter, Florida, USA.


The ascomycetous fungus Chrysosporium lucknowense C1 was developed as an efficient and versatile platform for high level protein production on a commercial scale, providing a strong alternative to well established industrial fungi, like Aspergillus niger and Trichoderma reesei. Strain and process improvement strategies of the original C1 isolate resulted in strains that are able to secrete large amounts of a complex mixture of (hemi-)cellulases. Re-sequencing and automated annotation of the wild type C1-genome using the latest sequencing and bioinformatics tools revealed that C1 is a rich source of (potential) industrial enzymes. These enzymes include oxido-reductases, proteases, esterases and hydrolases. In particular, the approximately 38 Mbp genome appeared to be very rich in genes encoding plant biomass hydrolyzing enzymes. Comparison of this plant cell wall degrading capacity with that of A. niger and T. reesei revealed interesting similarities and differences. C1 specifically differentiates itself from A. niger and T. reesei by the relatively large number of (glucurono-) arabinoxylan degrading enzymes. However, C1 and A. niger are similar with respect to the number of cellulases, while T. reesei has notably less cellulases. An overview of these and other C1 genome sequence data as well as some examples of the exploitation thereof will be presented.

abstract No: 


Full conference title: 

26th Fungal Genetics Conference
    • Fungal Genetics Conference 26th (2005)