RRMA is the RNA binding protein involved in posttranscriptional regulation of gene expression in Aspergillus nidulans. rrmA gene was identified as a suppressor of mutations in arginine/proline catabolic pathway. Independently RRMA protein was shown to bind to the 3’UTR of areA transcript (nitrogen positive regulator). Delta rrmA mutation results in slow growth phenotype and higher sensitivity to oxidative stress. Analysis of main antioxidant enzymes revealed different activity pattern during early development stages and under oxidative stress in delta rrmA strain comparing to the control strain. Microarrays analysis has shown that delta rrmA mutation results in changed stability of specific transcripts under conditions of oxidative stress and nitrogen starvation, e.g. the stability of deoxyhypusine synthase mRNA is decreased in delta rrmA. The enzyme is responsible for modification of eukaryotic initiation factor 5A (eIF5A) implicated in stress- induced translational repression. Our results indicate that RRMA plays important role in the mechanism of regulated degradation of specific mRNAs in response to specific signals.
Full conference title:
26th Fungal Genetics Conference
- Fungal Genetics Conference 26th (2005)