Using RNA8208;Seq data to improve the gene structure annotation of Aspergillus species

Ref ID: 18479

Author:

Gustavo C. Cerqueira, Marcus C. Chibucos, Christopher Sibthorp, Mark X. Caddick, Kazuhiro Iwashita, Gavin
Sherlock, Jennifer R. Wortman

Author address:

Broad Institute of MIT and Harvard, Cambridge, USA  
Institute for Genome Sciences, University of Maryland
School of Medicine, Baltimore, USA  
School of Biological Sciences, University of Liverpool, Liverpool, UK
National
Research Institute of

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The correct structural annotation of genes is fundamental to downstream functional genomics approaches. Genes
undetected by gene prediction algorithms, incorrect gene boundaries, misplaced or missing exons and wrongly
merged genes can jeopardize attempts to produce a comprehensive catalog of an organism’s metabolic
capabilities. We are currently working toward generating alternative and improved structural annotation of
Aspergillus species relevant to food industry and human health. Our approach consists of reconstituting transcript
sequences from RNA8208;Seq data and aligning those sequences against their respective loci in the reference genome.
Potential gene structure modifications are then validated by coding sequence conservation across closely related
species. In addition, novel algorithms were developed to deal with the challenges intrinsic to non8208;strand8208;specific
RNA8208;Seq, which represent the bulk of data currently available for the Aspergilli; and downstream analyses
leveraging the newly defined UTR regions were performed. The improved gene structure annotation of every
species associated to this effort will be freely available through the Aspergillus genome database site
(http://www.aspergillusgenome.org)  

Abstract Number: PR3.18

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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