Trichoderma glutamate decarboxylase plays an important role during germination and hyphae development

Ref ID: 18449

Author:

Lubos Niznansky, Svetlana Krystofova, Ludovit Varecka

Author address:

Slovak University of Technology Bratislava

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Glutamate decarboxylase (GAD) catalyzes glutamate decarboxylation to gamma8208;aminobutyrate (GABA) and is the
first enzyme of the GABA shunt cycle. Endogenous changes in GABA levels in fungi correspond with developmental
changes during conidiation and/or germination. It was observed previously that GAD activity in Trichoderma
atroviride F8208;534 changes during conidiation, early germination and stationary phase of submerged culture. A lack
of GAD activity during batch culture on sucrose as a sole carbon source lead to several aberrations in morphology
and development: increased formation of chlamydospore8208;like structures, longer lag8208;phase, lower germination
rate, higher number of bipolar germ tubes and increased hyphae branching. It has been demonstrated before that
in vitro analysis of GAD activity in Trichoderma decreased in presence of cyclosporine A, a calcineurin signaling
pathway inhibitor. Wild type strain in cultures containing various concentrations of cyclosporine A exhibited similar
phenotypic characteristics of the delta gad strains – increased bipolar germination and hyphae branching. The
similar effects of cyclosporine A have been known in other filamentous fungi (Neurospora, Aspergillus). Bipolar
germination and branching of delta gad strains remained unaffected in the presence of cyclosporine A indicating
that GAD could function as a downstream effector of calcineurin.

Abstract Number: PR1.10

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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