The transcriptional regulator RhaR of Aspergillus niger is involved in L-rhamnose catabolism and in degradation of Rhamnogalacturonan-I.

Ref ID: 18323

Author:

BS Gruben (1,2), M Zhou (1), A Wiebenga (1), J Ballering (2), RP de Vries (1,2)

Author address:

. (1) CBS-KNAW Fungal
Biodiversity Centre, Utrecht, The Netherlands; (2) Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht
University, The Netherlands.

Full conference title:

Asperfest 9

Abstract:

The Aspergillus niger genome contains a broad set of pectinolytic genes, encoding enzymes that act on the different substructures and
linkages of pectin. Previous studies demonstrated a complex regulation of these genes, but so far none of the regulators involved in
this process have been identified.
We identified the transcriptional activator RhaR is described that is mainly regulates the expression of genes involved in degradation
of Rhamnogalacturonan-I. Micro-array analysis revealed down-regulation of genes encoding exorhamnogalacturonases, α –
rhamnosidases, rhamnogalacturonan acetylesterases, an unsaturated rhamnogalacturonan hydrolase and a rhamnogalacturonan lyase in
the 916;rhaR strain compared to the reference strain on L-rhamnose. In addition, a gene encoding a putative pectin acetyl esterase, two
genes encoding putative β -1,4-galactosidases and one gene encoding a feruloyl esterase were also down-regulated in the disruptant.
RhaR also appears to regulate L-rhamnose catabolism as growth of rhaR disruptant strains on L-rhamnose was abolished and two
genes encoding putative L-rhamnose catabolic enzymes were down-regulated in the 916;rhaR strain.

Abstract Number: 74)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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