The role of Aspergillus nidulans myosin V, MyoE, in hyphal tip growth

Ref ID: 18451

Author:

Naimeh Taheri-Talesh, Yi Xiong, Berl R. Oakley

Author address:

The University of Kansas, Department of Molecular Biosciences  
The Ohio State University, Department of
Molecular Genetics

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Aspergillus nidulans has a single myosin V heavy chain gene which we designate myoE. myoE is not essential but
deletion of myoE causes abnormal hyphal morphology and branching anterior to the first septum. Tip extension in
myoE deletants is slower than in controls, but because the hyphal diameter is greater, the growth rate (increase in
volume/ unit of time) is only slightly reduced relative to myoE
+
controls. Growth is extremely slow in the absence
of MyoE and microtubules, but not completely inhibited. MyoE8208;GFP localizes to the Spitzenkörper (a vesicle supply
center) and to puncta in the cytoplasm that move bidirectionally. Treatments with inhibitors show that the
localization of MyoE at the Spitzenkörper is actin dependent but movement of some of the MyoE puncta in the
hyphae is actin independent. Time8208;lapse imaging of SynA, a v8208;SNARE, reveals that in myoE8710; cells vesicles no longer
localize to the Spitzenkörper. Photobleaching experiments with GFP8208;SynA show that in myoE
+
cells, the SynA
exocytic vesicles move rapidly from the non8208;bleached area of the hypha to the Spitzenkörper before incorporation
into the plasma membrane, whereas in myoE8710; cells they do not localize to the Spizenkörper. Rather they move
directly to the plasma membrane. Our data indicate strongly that MyoE function is required for the accumulation
of vesicles at the Spitzenköper which facilitates faster tip extension and a normal hyphal shape.

Abstract Number: PR1.17

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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