The major changes in the transcriptome during germination occur before isotropic growth and are not affected by the antifungal compound natamycin

Ref ID: 18510

Author:

M. R. Van Leeuwen,
T. T. Wyatt,
P. Krijgsheld,
E. A. Golovina,
H. Menke,
A. Dekker,
J. Stark,
H. Stam,
R.
Bleichrodt,
H. A. B. Wösten,
J. Dijksterhuis

Author address:

Applied and Industrial Mycology, CBS-KNAW/Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The
Netherlands.

Microbiology and Kluyver Centre for Genomics of Industrial Fermentation, Institute of
Biomembranes, Utrecht University,

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The transcriptome of Aspergillus niger was analysed during germination of conidia in the absence or presence of
the antifungal compound natamycin. Early germination was accompanied by a reduction in cellular microviscosity
and a drop in mannitol and trehalose levels. During 8 hours, normal germlings grew isotropically, followed by
polarized growth (germ tube formation) and performed 1 cycle of mitosis. With 3 μM natamycin, germination was
arrested at the stage of mitosis and germ tube formation and at 10 μM natamycin conidia even did not swell and
accumulated some mannitol.  
After 2 h of germination, upregulation in the transcriptome of natamycin treated conidia was very similar to
untreated conidia, especially protein synthesis, energy and rRNA translation. Correlation of the RNA profiles
between 28208; and 8 h indicated that all conidia change extensively, but highest in 10 μM natamycin, despite the lack
of morphological change. The RNA profiles of these cells were slightly more similar to dormant conidia then all
other samples. After 28208;  and 8 h, natamycin8208;treated cells contained increased numbers of expressed genes
compared to the controls in a dose8208;dependent manner. Transcripts of a number of protective compounds specific
for dormant conidia were highly accumulated in 8 hour old treated spores. These habits have developed secondary
after a large shift in the transcriptome.
All these cues point into the direction that germinating conidia that are confronted with adverse conditions have
no focus on strong vegetative development, but regain relatively high stress resistance, absence of growth and
prolonged survival.

Abstract Number: PR7.42

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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