The genetic basis of conidial pigmentation in Aspergillus niger

Ref ID: 18364

Author:

M. Arentshorst, T.R. Jørgensen, J. Park, A.M. van Welzen, G. Lamers, P.A.
vanKuyk, R.A. Damveld and A.F.J. Ram.

Author address:

. Institute of Biology Leiden, Leiden University, Molecular Microbiology and Biotechnology, Kluyver Centre
for Genomics of Industrial Fermentation, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.

Full conference title:

Asperfest 8

Abstract:

A characteristic hallmark of Aspergillus niger is the formation of black conidiospores. To understand the genetic basis of black spore formation, we have
identified four genes required for pigmentation by using a complementation approach. First, we characterized a newly isolated color mutant, colA, which
lacked pigmentation resulting in white conidia. Pigmentation of the colA mutant was restored by a gene (An12g03950) which encodes the A. niger
ortholog of the 4’-phosphopantetheinyl transferase protein (PptA). The loci giving rise to fawn, olive, and brown color phenotypes were identified by
complementation. The fawn mutant was complemented by the polyketide synthase A protein (PksA, An09g05730), the ovlA mutant by An14g05350
(OlvA) and the brnA mutant by An14g05370 (BrnA), the respective homologs of pksP/alb1, ayg1 and abr1 in A. fumigatus. Targeted disruption of the
four genes confirmed the complementation results. Epistasis was determined for pksA, olvA and brnA by constructing double mutants. This set of isogenic
color mutants is a useful tool to do classical genetic analyses in Aspergillus niger.

Abstract Number: 42)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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