The complexity of polyketide synthase OrsA action in Aspergillus nidulans

Ref ID: 18383

Author:

Jakob B. Nielsen, Michael L. Nielsen, Christian Rank, Marie L.
Klejnstrup, Paiman Khorsand-Jamal, Dorte M. K. Holm, Bjarne G. Hansen, Jens C. Frisvad, Thomas O. Larsen and Uffe H. Mortensen

Author address:

Center for
Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Sí¸ltofts Plads B221/B223, 2800 Kgs. Lyngby, Denmark.

Full conference title:

Asperfest 8

Abstract:

Polyketides (PKs) are chemically diverse molecules characterized by a broad spectrum of biological activities. Polyketide synthases (PKSs) are the main
engines in the synthesis of PKs, and in fungi the PKS assembles small subunits to polymers in an iterative manner. Mapping of PKs to PKSs are not
straight forward. The chemical architecture of the compounds produced depends on the primary structure and organization of the domains within the PKS
molecule, and a majority of the PK products are also influenced by other gene products typically clustered in vicinity of the PKS. Moreover, activation
of these clusters can be cumbersome, due to the clandestine regulation of the PK biosynthesis. One example is the gene cluster of PKS AN7909, orsA,
in Aspergillus nidulans, which produces orsellinic acid and several other products. This synthesis has been provoked by several strategies. Here we report
products that previously have not been linked to OrsA activity as well as cross-talk with the PKS AN7903 located approximately 20 kb downstream of
orsA. These new observations emphasizes the challenges there are in uncovering the PK metabolome in fungi and the molecular genetics herein, which
ultimately will facilitate rational engineering of the PK production apparatus.

Abstract Number: 61)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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