Author:
Percier-Lehebel P; De Prins S; Tima HG; Huygen K; Romano M; Denis O
Author address:
Scientific Institute of Public Health, Brussels, Belgium
Full conference title:
European Academy of Allergy and Clinical Immunology Congress 2017
Abstract:
Introduction: Aspergillus fumigatus is one of the most ubiquitous of the airborne saprophytic fungi, found both outdoors and indoors. Every day, humans inhale thousands of Aspergillus fumigatus conidia. In immunocompetent host, these conidia are eliminated by the innate immune system and a protective inflammation through the generation of Th1/Th17 responses. But in immuno-compromised hosts, Aspergillus fumigatus can induce serious diseases like aspergillosis. However, this aggressive fungi species can also activate detrimental Th2 responses in the lungs leading to the development of an allergic inflammation in such hosts.
Objectives: The early events inducing the allergic inflammation induced by Aspergillus fumigatus are actually not defined. The immune activating properties of this fungus species have been investigated in an asthma murine model to determine which receptor, signaling pathway are activated by conidia and identify which cytokines are crucial in this model.
Results: A model of lung allergic inflammation based on the transfer of dendritic cells pulsed in vitro with Aspergillus fumigatus was developed. Bone marrow-derived dendritic cells (BMDCs) from wild type or deficient mice were stimulated in vitro with Aspergillus fumigatus conidia and then transferred into wild type mice by intranasal administration. Fourteen days after the transfer, all mice were challenged with conidia intra-nasally and the lung inflammations were analysed three days later. This fungus species induced a strong lung Th2 response with a high recruitment of eosinophils in broncho-alveolar lavages and an increase of Th2 cytokine mRNA in lung homogenate. The transfer of BMDCs deficient for the expression of MyD88 induced a worsening of the allergic lung inflammation, which is specific of the Aspergillus fumigatus species.
Conclusions: These results indicate that the MyD88 pathway on BMDCs is able to control the allergic response generated by A. fumigatus via the recognition of compounds on Aspergillus fumigatus conidia.
Abstract Number: 1650
Conference Year: 2017
Link Conference abstract:
Conference abstracts, posters & presentations
-
Title
Author
Year
Number
Poster
-
v
C Gutierrez Perez1*, S Dhingra1,2, SM Kwansy3, TJ Opperman3, RA Cramer1
2022
93
-
v
PC Chatterjee1*, WC Chiu1, EBB Burgener2, PLB Bollyky3, JH Hsu1
2022
92
-
v
AS Serris1*, FU Uhel2, GMB Martin-Blondel3, OL Lambotte4, MEB Bougnoux5, EL Litvinova6, AO Ouedrani7, CR Rouzaud1, VB Bedarida8, JHR Raphalen9, FL Lanternier1,10
2022
91
-
v
ZA Lewis1, RB Meagher2*, S Ambati2, X Lin1
2022
90
-
v
QJ Choudhury1*, S Ambati2, X Lin1, ZA Lewis1, RB Meagher2
2022
-
v
M James1*, KW Liu1, E Vesley1, T Hohl2, RA Cramer1
2022
88
-
v
D Luptakova1, RH Patil1,2, V Havlicek1,2*
2022
87
-
v
EI Matthaiou1*, W Chiu1, C Conrad2, J Hsu1
2022
86
-
v
H Kamboj*, M Vijay, L Gupta, P Vijayaraghavan
2022
85
-
v
S Hartung1,2*, TNM Hoang1,2*, Z Cseresnyes3*, N Schuck1, K Rennert4,5, F Klos6, AS Mosig5,7, MT Figge3,8, M von Lilienfeld-Toal1,2
* Authors 1, 2 and 3 contributed equally
2022
84
