Targeted gene deletion and genome mining of the predicted Aspergillus carbonarius secondary metabolome.

Ref ID: 15888

Author:

Michael Praseuth1, Antonia Gallo2,
Scott E. Baker3 , Clay C. C. Wang1, and Kenneth S. Bruno3

Author address:

Department of Pharmacology and Pharmaceutical Sciences, University of Southern California,
School of Pharmacy, Los Angeles, California, USA. 3Chemical and Biological Process Development Group, Energy and Environment Directorate, Pacific
Northwest N

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

Interest in natural products produced by fungi has grown substantially in recent years. Available genome sequences of filamentous fungi have revealed
that these organisms contain the capacity to produce far more secondary metabolites than previously identified by conventional analysis. In large part,
the reason that these compounds remain uncharacterized is due to the lack of gene expression under typical laboratory conditions. One approach to
identifying novel compounds produced by fungi is heterologous expression of predicted secondary metabolite genes in a tractable model organism such
as Aspergillus nidulans. Although this has proved successful with some compounds, an analysis in the native organism could prove more fruitful in
determining all of the enzymes necessary to produce a given compound. For this reason we have developed a transformation protocol for transformation
in Aspergillus carbonarious strain ITEM 5010. We have successfully deleted the kusA orthologue in this strain using the hygromycin phosphotransferase
marker. In a subsequent transformation we deleted the orthologue of an Aspergillus niger gene responsible for naptha-pyrone production. Gene deletion
and overexpression will be used to identify genes responsible for production of secondary metabolites in this species.

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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