Systematic deletion analysis of Aspergillus nidulans kinase genes.

Ref ID: 15768

Author:

Colin P. De Souza, 1Shahr B. Hashmi, 1Aysha H. Osmani, 2Carol S. Ringelberg,
2Jay C. Dunlap and 1Stephen A. Osmani.

Author address:

1The Ohio State University, Columbus, OH, USA. 2Dartmouth Medical School, Hanover, NH, USA.

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

Phosphorylation mediated through kinase enzymes is important to the regulation of virtually all eukaryotic processes. In the filamentous fungi analysis
of these important regulatory enzymes has been limited to specific kinases identified through biochemical or genetic approaches or by sequence similarities
to kinases in other organisms. However, recent advances in gene targeting and the availability of pre-made deletion constructs makes possible the global
analysis of all kinases in Aspergillus nidulans. We report here the deletion and primary characterization of all protein kinase, histidine kinase and PI3/PI4
kinase encoding genes, totaling 130 deletions, in this model fungus. Each gene has been deleted and defined as either essential or not essential.
Non-essential haploid deleted strains have been tested for conditional phenotypes in response to numerous cellular stress conditions. In addition, for all
24 of the essential kinase genes, the terminal growth and cell cycle phenotype has been defined using heterokaryon rescue and microscopic analysis of
DAPI stained cells. This global analysis has confirmed the phenotypes of previously studied kinase genes and has expanded the number of kinase genes
that have now been characterized in A. nidulans by 82. The deleted strains have been deposited at the FGSC and provide a powerful resource for analysis
of processes regulated by phosphorylation in fungi. (Funded by NIH Program Project grant GM068087)

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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