Suppressors of the septum initiation network function on septation and conidiation in Aspergillus nidulans

Ref ID: 19312

Author:

Guowei Zhong, Wenfan Wei, Qi Guan, Zhaofei Ma, Hua Wei, Xushi Xu, Shizhu Zhang, Ling Lu

Author address:

Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology
Research Center for Microbiology; Jiangsu Key Laboratory for Molecular and Medical Biotechnology;
College of Life Sciences, Nanjing Normal University, N

Full conference title:

Asian Mycological Congress 2013 and the 13th International Marine and Freshwater Mycology Symposium

Date: 19 August 2014

Abstract:

Timely cytokinesis/septation is essential for hyphal growth and conidiation in Aspergillus
nidulans. Genetic analyses have shown that A. nidulans has components of the septum initiation network
(SIN) pathway; one of these, SEPH, is a key player for early events during cytokinesis. However, little is
known about how the SEPH kinase cascade is regulated by other components. Here, through UV
mutagenesis, 116 independent mutants were obtained that could restore cytokinesis in the absence of
sepH. Among them, the phosphoribosyl pyrophosphate synthetase family acts antagonistically against
the SIN so that the downregulation of AnPRS family can bypass the requirements of the SIN for septum
formation and conidiation. The transcription defect of the Anprs gene family accompanied with the
reduction of AnPRS activity causes the formation of hyper-septation as well as the restoration of
septation and conidiation in the absence of SEPH. Moreover, we demonstrated that AnPRS members are
able to form the heterodimers for functional interacting entities but they appear to contribute so unequally
that deletion of Anprs1 displays relatively normal septation, but deletion of either Anprs2 or Anprs3 is
lethal. In addition, two regulatory subunits of protein serine/threonine type 2A phosphatases (PP2A)-ParA
and PabA, whose orthologos are suppressors of SIN in Schizosaccharomyces pombe had been found to
be required for conidiation and septation. Deletion of parA but not pabA caused the hyper-septation in
AMC 2013 & 13th IMFMS August 19-23, 2013 Beijing, China
36
hyphal cells, especially in conidiophore cells. Therefore, SIN, as a major signaling pathway in regulating
cytokinesis, might have to work together with multiple other protein complexes.

Abstract Number: s3-03

Conference Year: 2013

Link to conference website: NULL

New link: NULL

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