Sequencing the Black Aspergilli species complex

Ref ID: 18326

Author:

Kuo, Alan , Asaf Salamov , Scott Baker , and Igor Grigoriev

Author address:

DOE Joint Genome Institute, CA,
USA. Pacific Northwest National Lab, WA, USA. akuo@lbl.gov.

Full conference title:

Asperfest 8

Abstract:

The ~15 members of the Aspergillus section Nigri species complex (the “œBlack Aspergilli”) are significant as platforms for bioenergy and bioindustrial
technology, as members of soil microbial communities and players in the global carbon cycle, and as food processing and spoilage agents and agricultural
toxigens. Despite their utilityand ubiquity, themorphological and metabolic distinctiveness of the complex’s members, and thus their taxonomy, is poorly
defined. We are using short read pyrosequencing technology (Roche/454 and Illumina/Solexa) to rapidly scale up genomic and transcriptomic analysis
of this species complex. To date we predict 11197 genes in Aspergillus niger, 11624 genes in A. carbonarius, and 10845 genes in A. aculeatus. A.
aculeatus is our most recent genome, and was assembled primarily from 454-sequenced reads and annotated with the aid of > 2 million 454 ESTs and
> 300 million Solexa ESTs. To most effectively deploy these very large numbers of ESTs we developed 2 novel methods for clustering the ESTs into
assemblies. We have also developed a pipeline to propose orthologies and paralogies among genes in the species complex. In the near future we will apply
these methods to additional species of Black Aspergilli that are currently in our sequencing pipeline.

Abstract Number: 3)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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