Secretion of Client Proteins in Aspergillus

Ref ID: 18524

Author:

Fernando Segato,
Andre Damasio,
Thiago Gonçalves,
Rosymar de Lucas,
Fábio Squina,
Stephen Decker,
Rolf
Prade

Author address:

Brazilian Bioethanol Science and Technology Laboratory – CTBE  
São Paulo University – USP  Â 
National
Renewable Energy Laboratory – NREL  
Oklahoma State University  

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Production of pure and high8208;yield client proteins is an important technology that attends the needs for industrial
applications of enzymes as well as basic experiments such as protein crystallization.   Client protein expression
platforms are available in Escherichia coli and the methylotrophic Pichia pastoris that result in proteins released to
the intracellular cell extract and extracellular medium, respectively.   Fungi are utilized in industrial protein
production because of their ability to secrete large quantities.   In this study we engineer a high8208;expression8208;
secretion vector, pEXPYR that directs proteins towards the extracellular medium in two Aspergillii host strains,
examine the effect of maltose overexpression, production time and pH8208;dependent protein stability in the
medium.  We describe five client proteins that accumulated 508208;100 mg of protein per liter and only one protein
was secreted at low quantities.  We also test a recyclable genetic marker that allowed secretion of multiple client
proteins, enabling the design of an enzyme activity set.

Abstract Number: PR8.29

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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