Secrelection: A Novel Fungal Expression System For Selection Of Secreted Enzymes.

Ref ID: 15922

Author:

Robbert Damveld 1, Brenda Vonk 1, Cees Sagt 1, Hildegard
Menke 1, Thibaut Wenzel

Author address:

1DSM Biotechnology Center

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

Modern biotechnology has generated an impressive set of molecular tools: for instance the ability to generate large sets of error prone mutant libraries
or cDNA libraries. When these libraries are expressed in a host (e.g. Aspergillus niger) not all strains produce a secreted protein. This is mainly dependent
on the quality of the library. Here we describe a novel expression system that was developed by using genome expression profiling under different
conditions. We were able to identify promoters that fit the required expression profile. These promoters were both up regulated during protein secretion
and were not expressed during overexpression of intracellular proteins. By making use of transcriptomics for useful promoter identification, we were able
to generate reporter construct(s) that allow us to easily select clones that secrete proteins. This technology can speed up novel protein discovery
significantly. Additionally we have shown this approach is not limited to fungi but can also be applied to other production organisms.

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster