Role of Candida in immunomodulations in patients suffering from pemphigus

Ref ID: 19629

Author:

Shukla Das*. SA Dar,VG Ramachandran, N Bhattacharya, B Banerjee,V Sood and A Banerjea

Author address:

Department of Microbioloy, UCMS & GTB Hospital, Dilshad Garden, Delhi, 110 095, India

Full conference title:

Society for Indian Human and Animal Mycologists 2014

Date: 10 January 2014

Abstract:

Pemphigus, an autoimmune skin disease characterized by intraepithelial blisters, induced by autoantibodies directed against
desmosomal adhesion proteins. B cells and T cells play a major role in regulation and induction of specific immune response
against infectious agents. Role of superantigens and Candida recall antigens in provoking polyclonal activation of T cells
including autoreactive T cells is still unknown. The study was initiated to investigate response of T cells (CD4+/CD8+) to
Candida antigens and the role of memory cells CD45RO & CD45RA in modulating the autoantibody response in pemphigus
patients as compared to healthy individuals. 10 ml blood was collected from 25 adult patients of pemphigus and 10 healthy
individuals. PBMCs were separated and 1 x 106 cells were incubated with 1-10 ug/ml PHAM, 10-100 ng/ml C. albicans in a 12
well cell culture plate with RPMI1640 at 37°C & 5% C02 x 72 hrs. Cells were washed and stained with appropriate monoclonal
antibodies & scanned with BD FACS Calibur. Patients of pemphigus had decreased level of naive CD45RA + RO- cells as
compared to healthy. CD3+/CD4+ T cells were elevated and isoform switch over from CD45RA to CD45RO indicates a rise in
memory cells due to constant Candida antigen trigger from the skin.
’Corresponding author
E-mail:shukladas_123@yahoo.com

Abstract Number: OP-010

Conference Year: 2014

Link to conference website: http://www.siham2014.com

New link: NULL

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