Rationale: Sensitization to Aspergillus spp. is associated with severe asthma, as well as
Methods: Patients with allergic bronchopulmonary aspergillosis were recruited from within the Vanderbilt system. Peripheral blood mononuclear cells were isolated, and IgE specific B-cells were immortalized using standard electrofusion techniques.
Results: Approximately half of
Conclusions: The Aspergillus fumigatus extract currently used in immunotherapy in the US is highly variable in relation to the content of component allergens. This degree of variability is almost certain to affect the efficacy of these reagents. Human monoclonal IgE represents a novel tool for evaluation of
Full conference title:
- AAAAI 2017 (73rd)