Relationship of serological reactivity to cytoplasmic extracts from spores of Ganoderma applanatum and commercial extracts of indoor, mitosporic fungi, and farm animal allergens among Puerto Rican subjects

Felix E. Rivera-Mariani, PhD1 , Hayat H. Srour, MS1 , Joshua K. Baguley, MS1 , Shandra V. Bellinger, MS1 , Ariel J. Stateman, MS1 , and Benjamin Bolanos, PhD2

Author address: 

1 College of Biomedical Sciences, Larkin University, Miami, FL, 2 University of Puerto Rico, San Juan, PR, Puerto Rico.


RATIONALE: Fungal spores with allergenic potential are a predominant biological component in the atmosphere of Puerto Rico. Spores of basidiomycete fungi, such as Ganoderma spp, often outnumber the atmospheric concentration of mitosporic fungi and pollen from trees and grass. Nevertheless, extracts from basidiomycetes are not currently available for serological testing and knowledge of the relationship of reactivity with commercial extracts is limited.

METHODS: Spearman correlation matrices were generated, on an archived dataset, to identify relationships between reactivity to G. applanatum spore’s cytoplasmic extracts tested in ELISA (crude) and Western blot (19, 24, 33, 45, 56, 75, and 81 kDa) with reactivity to indoor, pests, farm animals, and pollen allergens commercial extracts. Also, principal component analysis was employed to identify clusters of variables explaining inter-subjects variabilities in reactivities.

RESULTS: Only the reactivity to dog danger was highly correlated with the crude extract (r 50.48, p < 0.001), but the 45 and 75 kDa polypeptides were correlated with reactivities to mitosporic fungi (Alternaria, Aspergillus, Fusarium, Cladosporium), Mucor, pests (American cockroach, mouse, rat), and farm animal danders (chicken, horse) (r 5 .30 to 57, p 5 0.04 to < 0.001). G. applanatums polypeptides (except the 19 kDa) clustered together in explaining 21% of the variability among the subjects’ reactivities.

CONCLUSIONS: These results suggests that reactivity to mitosporic fungi, indoor allergens, and allergens from farm animals pose a respiratory health risk from exposures to G. applanatum fungal spores. Our findings also further support the relevance of G. applamatum as an important outdoor allergen among the Puerto Rico population.


abstract No: 


Full conference title: 

American Academy of Allergy, Asthma & Immunology 2019
    • AAAAI 2019