Rapid identification of Candida species by MALDI-TOF technology

Ref ID: 17779

Author:

B. Nonnemann*, S.H. Hartzen, M. Tvede, T. Bjarnsholt

Author address:

(Esbjerg,
Copenhagen, DK)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

Objectives and background: Rapid identification of yeast isolates is
important in order to adjust the antifungal treatment and to reduce
patient morbidity and mortality.
In this study we investigate whether Matrix assisted laser desorption/
ionization time of flight mass spectrometry (MALDI-TOF MS) can (i)
replace current routine methods in first line identification of Candida,
(ii) examine differences in generated results by two mass spectrometry
instruments situated in two routine laboratory settings, (iii) measure
time from entry of sample in the instrument to results. In East Denmark,
the Microflex LT (Bruker Daltonics, Germany) and in West Denmark,
the Vitek MS (bioMe’rieux, France).
Method: Retrospective analysis of Candida isolates with identification
in parallel by two MALDI-TOF instruments and API. API 20 C AUX
(bioMe’rieux, France) was used as the standard method.
Results: Ninety-three isolates were examined on Microflex LT with
the Biotyper 2.0 software according to the manufacturer¢s manual.

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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