Random amplified polymorphic DNA as a novel method for making a link between the Aspergillus clinical and hospital enviromental isolates

Ref ID: 19555

Author:

A Namaki1, K Diba2*, Z Khorshidvand2, D Jabbari2

Author address:

1Arefian General Hospital, Urmia, Iran
2Department of Medical Mycology, School of Medicine, Urmia University of Medical Sciences

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Aspergillosis is known to be an airborne infection and the nosocomial infections are associated
with constructions and increased dust loads in hospital indoors. Our main object was to find the
environmental sources of Aspergillus species causing hospital acquired infections.
Methods:
The clinical and environmental samplings were performed during 18 months from spring 2010 to
summer 2011 in an educational hospital. A morphological diagnosis was used including media culture,
for the first identification of all isolated Aspergillus species. For the random amplified polymorphic
DNA (RAPD) assay, extraction of DNA was performed using manual phenol-chloroform method
followed by PCR with six random primers. The results of RAPD were compared between the clinical
Aspergillus isolates and hospital indoor isolates.
Results:
Application of primer in RAPD was resulted different patterns for the pairs 16, 36 and 237, but same
pattern for the pair of 31 including; case and environmental Aspergillus isolates. P2 resulted identical
patterns for Aspergillus groups 16, 31 and 237. Comparison of clinical and environmental revealed
the pairs 32 and 45 are only Aspergillus strains with identical RAPD patterns. The Aspergillus
isolated from the case no(32) BAL sample was completely similar to that of relevant air conditioner
sample in RAPD analysis. Also, banding patterns of A. flavus isolated from case no(45) sinus
discharge was similar to those of wall swabs.
Conclusion:
The hospital sources for the Aspergillus clinical isolates included air condition and walls and RAPDPCR
analysis can play a trivial role in finding the hospital sources of Aspergillus clinical isolates.

Abstract Number: 82

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster