Quantitative galactomannan detection in comparison to real-time PCR method in diagnosing invasive aspergillosis

Ref ID: 19383

Author:

M. Golas,1 K. Piskorska,2 M. A. Sikora,3 I. Netsvyetayeva,2
B. Sulik-Tyszka4 and E. Swoboda-Kopec3

Author address:

1Medical University of Warsaw, Warsaw, Poland; 2Medical
University of Warsaw, Department of Medical Microbiology,
Warsaw, Poland; 3Medical University of Warsaw, Department of
Dentistry Microbiology, Warsaw, Poland and 4Central Clinical
Hospital,

Full conference title:

6th Trends in Medical Mycology 2013

Date: 11 October 2014

Abstract:

This work was supported by the Polish State Committee for Scientific
Research (grant no. N N401 042738)
Objectives Invasive aspergilosis (IA) is an acute infection with a
mortality rate of almost 70%. An etiological factor in more then
90% of cases is Aspergillus fumigatus. The most common clinical
manifestation is lung aspergilosis – 75% of all IA cases, rhinosinusitis
(infection of the nasal mucosa and nasal sinuses) – 5-10%, dis-
seminated multiorgan – 25%, IA with an affected central nervous
system makes out 10-40% of all cases. Traditional methods: as a
“œgolden standard”in the diagnostic of invasive aspergilosis until
now, remains the culture of a strain from the sample clinical mate-
rial and identification of fungi by histopathology. As supportive
tests can be used: detection of galactomannan antigen and fungal
DNA in bronchioalveorial fliud or in serum. The aim of the study
was to compare two metods: immunoenzymatic detection of galac-
tomannan antigen (Platelia-Aspergillus Ag, BioRad, Hercules, USA)
and molecular method Real-Time PCR for the detection of Aspergil-
lus spp. genomic DNA (MycAssayTM Aspergillus, Myconostica,
Manchaster, UK).
Methods Serum samples were collected through routine serological
diagnostic procedures from patients hospitalized in the Institute of
Transplantation Medicine. Patients were selected retrospectively on
the base of availability of serum samples (at least two samples from
one patient), clinical sympthoms of IA and the results of culture
method. The tests were performed using serum samples of 2 groups
of patients: patients with clinical symptoms of invasive aspergillosis
and patients with positive reasult of galactomannan antigen test.
Both methods were conducted according to manufacturer protocols.
Results In group of patients with positive galactomannan antigen
test only 50% were positive in Real-Time PCR method. Serum sam-
ples collected from patients with clinical symptoms of invasive aspergillosis were all negative for galactomannan antigen. However,
in MycAssay test all collected serum samples of one patients were
positive. In the rest of the serum samples from other patients the
obtained results were both positive and negative. None of the speci-
mens from this group gave the unequivocal confirmation of the
infection.
Conclusions None of the compared method might be used as a sin-
gle indicator of Invasive Aspergillosis.
The result of MycAssay were frequently unequivocal and difficult
for interpretation.
The immunoassay test in comparison to MycAssay provided the
divergent results.

Abstract Number: p052

Conference Year: 2013

Link to conference website: NULL

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