Pleiotropic effects of farA disruption in Aspergillus oryzae

Ref ID: 18277

Author:

Sharon Marie Garrido, Akira Watanabe, Takahiro Shintani,

Author address:

Katsuya Gomi Laboratory of Bioindustrial Genomics, Graduate
School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

Full conference title:

Asperfest 9

Abstract:

This study reports on the pleiotropic effects of farA disruption- regulation of genes that at first sight do not seem to be influenced by
farA gene in Aspergillus oryzae. It has already been reported that FarA is a Zn(II) 2Cys6 transcription factor which up-regulates genes
required for growth on fatty acids in Aspergillus nidulans (Hynes et al. 2006) and it is also known to regulate cutinase genes in
Fusarium solani and A. oryzae (Li et al. 1997; Li et al. 2002; Garrido et al, 2012). However, its effect on the regulation of other
metabolic genes in A. oryzae has not yet been reported. Microarray analysis was conducted between the wild-type (WT) and the farA
disruptant induced with oleic acid. Results confirmed by qRT-PCR showed that a number of genes encoding ribosomal proteins such
as 60S ribosomal proteins L18 and L19, and 40S ribosomal proteins L6 and S16 were down- regulated in the disruptant as compared
to the WT. It could be implicated that ribosomal gene expressions during fatty acid metabolism may be FarA dependent and farA
disruption affects directly or indirectly the ribosomal biogenesis. Furthermore, metabolic genes up-regulated in the disruptant and
other metabolic genes affected only by oleic acid are also discussed in this study. Results are relevant for understanding more the
complexity of transcriptional regulatory networks of filamentous fungi.

Abstract Number: 23)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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