Peroxisomal localization of siderophore biosynthesis in Aspergillus ssp

Ref ID: 18290

Author:

Mario Gruendlinger
, Michael Hynes
and Hubertus Haas

Author address:

Division of Molecular Biology, Medical Univerisity, Innsbruck
Department of Genetics, University of Melbourne, Australia

Full conference title:

Asperfest 9

Abstract:

Virtually all organisms require iron as an essential nutrient. Siderophores, low molecular mass, iron-specific chelators, play a central
role in iron acquisition, iron storage and virulence of various phyto- and animal- pathogenic fungi. However, the subcellular
localization of siderophore biosynthesis is unknown. A. fumigatus and A. nidulans produce two major siderophores: extracellular
triacetylfusarinine C (TAFC) for iron acquisition and intracellular ferricrocin (FC) for iron storage. Interestingly, two TAFC
biosynthetic enzymes, SidH (cis-anhydromevalonyl CoA-hydratase) and SidF (N5-hydroxyornithine:cis–anhydromevalonyl CoA-N5
transacylase), possess putative peroxisomal targeting signals type 1 (PTS1), which are highly conserved in their orthologs of
Aspergillus ssp. . Using N-terminal GFP- tagging of SidH and SidF, we could show that the TAFC biosynthesis is, in part, localized in
peroxisomes. Additionally SidH were localized in peroxin mutant strains of A. nidulans to confirm PTS1 dependent import. Peroxins
are proteins critical for peroxisome biogenesis (e.g. PexC) or protein targeting into peroxisomes (e.g. PexE). Furthermore peroxin
mutant strains were compared to the wild type with respect to siderophore biosynthesis and growth rate during iron-replete and iron
depleted conditions to show the role of peroxisomes in iron acquisition. This is the first description of peroxisomal localization of
siderophore biosynthetic steps.

Abstract Number: 40)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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