PCR for diagnosis of tuberculosis and pulmonary mycoses in México

Ref ID: 18406

Author:

Rubén Garrido , Elisa Barrera, Karla Cabada.

Author address:

Department of Neumology and
Thoracic Surgery, Specialty Medical Center, Ciudad Juárez, Chihuahua, Mexico;
Institute of Biomedical Sciences, Autonomous University of Juarez City, Ciudad
Juárez, Chihuahua, Mexico

Full conference title:

European Respiratory Congress

Abstract:

Introduction: Tuberculosis (TB) and pulmonary mycoses are a public health
problem in the north of Mexico. We use polymerase chain reaction (PCR) for
diagnosis of TB in patients with a negative baciloscopy. This method also allows
us to differentiate between other Mycobacterium and microorganisms like the case
of Histoplasma capsulatum, Coccidioides immitis and Aspergillus fumigatus.
Objective: The identification of Mycobacterium tuberculosis in patients showing
clinical and radiological findings with negative baciloscopies and its association
with pulmonary mycosis.
Methods: Symptomatic patients with high pulmonary TB probability were selected
by a complete physical exam, clinical history, thoracic x rays and baciloscopy. In
patients with a negative baciloscopy an sputum sample was taken or a Bronchialalveolar
lavage to identify the presence of Mycobacterium, Histoplasma, Coccidioides
and Aspergillus by PCR. Adequate treatment was administered according
to the results obtained.
Results: A total of 23 patients were analyzed. The presence of Mycobacterium
tuberculosis was found in 9 cases. In 5 subjects there was of Aspergillus fumigates
and in 4 cases of both. In 2 cases the presence of Mycobacterium tuberculosis,
Histoplsma capsulatum, Coccidioides immitis were associated. Three samples were
negative for all those microorganisms.
Conclusions: PCR’s sensibility and specificity increases the diagnosis of TB and
mycoses cases. It is a magnificent tool when baciloscopy is negative generating the
need for use in the everyday medical practice. The use of innovative techniques
for early diagnosis and treatment of frequent pulmonary disease in this region is
imperative.

Abstract Number: P1823

Conference Year: 2011

Link to conference website: http://www.ers-education.org/ersMade/abstract_print_11/files/Abstract_book_2011.pdf

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