Molecular epidemiology of Aspergillus nosocomial infections a novel restriction enzyme

Ref ID: 19500

Author:

K Diba1, K Makhdoomi2, A Namaki3*, D Jabbari1

Author address:

1Medical Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
2Nephrology, Imam Educational Hospital, Urmia University of Medical Sciences, Urmia, Iran
3Surgery, Arefian General Hospital, Urmia, Iran

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
The aim of this study was searching the frequent Aspergillus species causing hospital aquired
infections and the environmental sources for Aspergillus infectiions in hodpital indoors.
Methods:
The subject of our study included bronchial fluids and sputum were collected from the hospitalized
patients with acute respiratory symptoms. For the environmental study, some specimens were
collected from air and environment surfaces. A morphological study was firstly performed including;
growth characteristics and microscopic features of Aspergilus species on mycological media.
For the confirmation of Aspergilus isolates which similarly found in clinical and environmental
sources, PCR-restriction fragment lenght polymorphism using a novel restriction enzyme Mwo I
and the molecular technique of random amplified polymorphic DNA (RAPD) were carried out for
searching the hospital aspergilus sources.
Results:
Totally of 102 fungal isolates, including Candida species 82 (80%), Aspergillus spp.20 (19.6%)
and the other fungi 2 (0.4%). Among the clinical isolates, Aspergillus flavus (8.4%), Aspergillus
fumigatus (4.2%) and Aspergillus niger (2.8%) were identified, as well as environmental Aspergillus
isolates Aspergillus flavus (19.4%), Aspergillus niger (6.5%) and Aspergillus fumigatus (6.5%).
Conclusion:
Comparing the clinical and environmental findings, 2 of 11 clinical Aspergillus isolates were matched
with environmental isolates from ventilation systems and wall surfaces, using RAPD but the other
environmental sources were not confirmed.

Abstract Number: 28

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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