Ref ID: 18433
Author:
STEPHEN DOLAN, Rebecca Owens, Lorna Gallagher, Kevin Kavanagh
& Sean Doyle
Author address:
National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland
Full conference title:
Society for General Microbiology
Date: 26 March 2014
Abstract:
Gliotoxin, a redox active molecule, is secreted by Aspergillus fumigatus
and plays a significant role in mediating its virulence. Biosynthesis and
secretion of this molecule is encoded by a 13 gene cluster and the
function of a number of genes within this cluster is still unknown. The
purpose of this work was to investigate the function of a gene, gliK, in the
cluster. Gliotoxin presence or absence in an A. fumigatus strain lacking gliK
(916;gliK) was determined by RP-HPLC, MALDI-ToF and LC-MS analysis. A.
fumigatus 916;gliK was found to be deficient in both gliotoxin biosynthesis
and secretion. A number of mutant-specific secreted metabolites, possibly
biosynthetic intermediates, were detectable by RP-HPLC and LC-MS.
A. fumigatus 916;gliK was shown to be highly sensitive to both exogenous
gliotoxin and hydrogen peroxide. unexpectedly, addition of exogenous
gliotoxin significantly relieved (p
2
O2
-induced oxidative stress in a dosedependent manner (0-10 μg/ml). Also, organic extracts derived from A.
fumigatus, which contained gliotoxin, significantly inhibited (p gliK mutant
growth, while the reverse was not found. Overall, our results confirm
that gliK plays a key role in gliotoxin biosynthesis and self-protection,
and demonstrate that gliotoxin presence protects A. fumigatus against
hydrogen peroxide-induced oxidative stress
Abstract Number: CMM/07
Conference Year: 2012
Link to conference website: http://www.sgm.ac.uk/meetings/SGM%20Dublin%202012_Abstracts.pdf
New link: NULL
Conference abstracts, posters & presentations
-
Title
Author
Year
Number
Poster
-
v
C Gutierrez Perez1*, S Dhingra1,2, SM Kwansy3, TJ Opperman3, RA Cramer1
2022
93
-
v
PC Chatterjee1*, WC Chiu1, EBB Burgener2, PLB Bollyky3, JH Hsu1
2022
92
-
v
AS Serris1*, FU Uhel2, GMB Martin-Blondel3, OL Lambotte4, MEB Bougnoux5, EL Litvinova6, AO Ouedrani7, CR Rouzaud1, VB Bedarida8, JHR Raphalen9, FL Lanternier1,10
2022
91
-
v
ZA Lewis1, RB Meagher2*, S Ambati2, X Lin1
2022
90
-
v
QJ Choudhury1*, S Ambati2, X Lin1, ZA Lewis1, RB Meagher2
2022
-
v
M James1*, KW Liu1, E Vesley1, T Hohl2, RA Cramer1
2022
88
-
v
D Luptakova1, RH Patil1,2, V Havlicek1,2*
2022
87
-
v
EI Matthaiou1*, W Chiu1, C Conrad2, J Hsu1
2022
86
-
v
H Kamboj*, M Vijay, L Gupta, P Vijayaraghavan
2022
85
-
v
S Hartung1,2*, TNM Hoang1,2*, Z Cseresnyes3*, N Schuck1, K Rennert4,5, F Klos6, AS Mosig5,7, MT Figge3,8, M von Lilienfeld-Toal1,2
* Authors 1, 2 and 3 contributed equally
2022
84
