Localization Analysis of P8208;bodies and Stress Granules under Different Stress Conditions in the Filamentous Fungus Aspergillus oryzae

Ref ID: 18462


Hsiang-Ting Huang, Jun-ichi Maruyama, Katsuhiko Kitamoto 

Author address:

University of Tokyo

Full conference title:

11 th European Conference on Fungal Genetics


Recently, it has become apparent that one aspect of posttranscriptional regulation of gene expression acts on the
remodeling of translating mRNAs from polysomes into non8208;translating mRNPs (messenger ribonucleoproteins). P8208;
bodies (processing bodies) and stress granules are two types of cytoplasmic mRNP granules that are widely
observed in eukaryotes. Both of them are implicated in the posttranscriptional regulation of gene expression when
cells are exposed to stresses. However, these structures in filamentous fungi including Aspergillus oryzae have not
yet been elucidated in detail. In this study, the well8208;known components of P8208;bodies and stress granules were
tagged with fluorescent proteins and analyzed in A. oryzae under various stress conditions. AoDcp28208;EGFP and
AoEdc38208;EGFP8208;labeled P8208;bodies were observed in the cytoplasm under normal growth condition, and were further
induced in response to stresses. AoPab18208;EGFP and AoPub18208;EGFP showed a dispersed distribution under normal
growth condition, and the aggregation was induced to form stress granules under stresses. Most of the stress
granules were colocalized or closely associated with P8208;bodies.   Additionally, it has known that A. oryzae AoSO
protein, a homolog of the Neurospora crassa SO, accumulates at septal pore in response to stresses. AoPab1 was
also colocalized with the AoSO cytoplasmic foci induced by the high temperature stress, while the AoSO
accumulation at the septal pore did not accompany with the AoPab1. The colocalization of the AoSO cytoplasmic
foci with stress granules suggested that AoSO may have a novel role in the regulation of mRNP granules

Abstract Number: PR1.46

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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