Ref ID: 19506
M Lakhtin1, A Bajrakova1, V Lakhtin1*, S Afanasiev1, V Aleshkin1
1Medical Biotechnology, G.N. Gabrichevsky Research Institute for Epidemiology & Microbiology, Moscow,
Full conference title:
6th Advances Against Aspergillosis 2014
Probiotic bacterial lectins (PBL) are members of new class of antimicrobials, synergistic metabolic
imitators of probiotics, perspective ingredients of synbiotics. The aim is to study influence of PBL
towards heterobiofilms involving human Ascomycetes.
PBL from industrial strains of probiotic strains of lactobacilli and bifidobacteria of human origin
(acidic and basic PBL as four preparations), freshly obtained and identified fungal isolates from
patients, prolonged growth of isolates on agar in Sabouraud medium in the presence of discs-PBL
in stress conditions at low temperatures, analysis of computer photos.
1. Mosaic distribution of discs-PBL influenced forming and distribution of final “œYeast like
Candida – filamentous fungi” massives as assymmetric landscapes of “œLacunas – Hills” type.
Aspergillus or Penicillium coisolates protected residual Candida biofilm at both steps of colonia
forming and further growth as upper layer resulting in decreasing summary Lacunas regions in
2. Mycobiota biorhythm of changing PBL-initiated symbiotic landscapes was observed.
Aspergillus colony forming was localized along borders between Lacunas and Candida residual
biofilms. Primary Aspergillus brown pigment population (later as black one) was spread Candida
sensor thick border along gradient of increasing Candida mass in direction of formal centers of
local communicative bodies. Similarly to Aspergillus (but later), Penicillium formed independent
compact homogenous massives (of age depended on varying green color for separately placed
massives) on free exposed residual Candida biofilms. Aspergillus or Penicillium layers protected
Candida biofilms from further lysis.
3. Lacunas were completely free of microbiota for a long time and served as reservoirs of
antimicrobials broadening in spectrum upon prolongation. Three months later, the only seldom spots
(1-3 of 5-8 mm in diameter, per plate) of one type non-pigmented conidial fungus were registered. 4.
Disc’s surface and closed to discs events were non-significantly depended on PBL type.
Results further develop our conception of communicative bodies for cases of Ascomycetes
communities similar to “œYeast like fungi – Filamentous fungi” heterofungal biofilms. PBL may serve
as regulators of earlier mycobiota biofilm forming as well as destructors of Candida based biofilms
involving later Aspergillus or Penicillium. Anti-candidosis agents and factors may be effective
against aspergillosis. PBL-initiated primary and secondary contents of Lacunas can serve as regions
of directed, controlled, diagnostic, prognostic and constructed antimicrobial events, natural locally
different sources of synergistic suitable panels and mixtures of antimicrobials depending on mosaics
of surrounding discs contributors. “œLacunas – Biofilms” can serve as important informative model of
functioning relatively pathogenic fungal communities in the presence of constructed synbiotic noncellular
ingredients and alternatives. New antifungal strategies (for example, against aspergillosis)
are proposed, and new mechanisms of antimicrobial actions (for example, antimycotic ones) of
symbiotic biotope compartment in human are predicted.
Abstract Number: 34
Conference Year: 2014
Link to conference website: http://www.AAA2014.org
New link: NULL
Conference abstracts, posters & presentations
C Gutierrez Perez1*, S Dhingra1,2, SM Kwansy3, TJ Opperman3, RA Cramer1
PC Chatterjee1*, WC Chiu1, EBB Burgener2, PLB Bollyky3, JH Hsu1
AS Serris1*, FU Uhel2, GMB Martin-Blondel3, OL Lambotte4, MEB Bougnoux5, EL Litvinova6, AO Ouedrani7, CR Rouzaud1, VB Bedarida8, JHR Raphalen9, FL Lanternier1,10
ZA Lewis1, RB Meagher2*, S Ambati2, X Lin1
QJ Choudhury1*, S Ambati2, X Lin1, ZA Lewis1, RB Meagher2
M James1*, KW Liu1, E Vesley1, T Hohl2, RA Cramer1
D Luptakova1, RH Patil1,2, V Havlicek1,2*
EI Matthaiou1*, W Chiu1, C Conrad2, J Hsu1
H Kamboj*, M Vijay, L Gupta, P Vijayaraghavan
S Hartung1,2*, TNM Hoang1,2*, Z Cseresnyes3*, N Schuck1, K Rennert4,5, F Klos6, AS Mosig5,7, MT Figge3,8, M von Lilienfeld-Toal1,2
* Authors 1, 2 and 3 contributed equally