Lectins of human probiotic bacteria influence biorhythmic landscapes of human biofilms “Candida – Aspergillus / Penicillium ”: resistant in time lysis regions possessing synergistic broadened antimicrobial spectrum

Ref ID: 19506

Author:

M Lakhtin1, A Bajrakova1, V Lakhtin1*, S Afanasiev1, V Aleshkin1

Author address:

1Medical Biotechnology, G.N. Gabrichevsky Research Institute for Epidemiology & Microbiology, Moscow,
Russia

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Probiotic bacterial lectins (PBL) are members of new class of antimicrobials, synergistic metabolic
imitators of probiotics, perspective ingredients of synbiotics. The aim is to study influence of PBL
towards heterobiofilms involving human Ascomycetes.
Methods:
PBL from industrial strains of probiotic strains of lactobacilli and bifidobacteria of human origin
(acidic and basic PBL as four preparations), freshly obtained and identified fungal isolates from
patients, prolonged growth of isolates on agar in Sabouraud medium in the presence of discs-PBL
in stress conditions at low temperatures, analysis of computer photos.
Results:
1. Mosaic distribution of discs-PBL influenced forming and distribution of final “œYeast like
Candida – filamentous fungi” massives as assymmetric landscapes of “œLacunas – Hills” type.
Aspergillus or Penicillium coisolates protected residual Candida biofilm at both steps of colonia
forming and further growth as upper layer resulting in decreasing summary Lacunas regions in
plates.
2. Mycobiota biorhythm of changing PBL-initiated symbiotic landscapes was observed.
Aspergillus colony forming was localized along borders between Lacunas and Candida residual
biofilms. Primary Aspergillus brown pigment population (later as black one) was spread Candida
sensor thick border along gradient of increasing Candida mass in direction of formal centers of
local communicative bodies. Similarly to Aspergillus (but later), Penicillium formed independent
compact homogenous massives (of age depended on varying green color for separately placed
massives) on free exposed residual Candida biofilms. Aspergillus or Penicillium layers protected
Candida biofilms from further lysis.
3. Lacunas were completely free of microbiota for a long time and served as reservoirs of
antimicrobials broadening in spectrum upon prolongation. Three months later, the only seldom spots
(1-3 of 5-8 mm in diameter, per plate) of one type non-pigmented conidial fungus were registered. 4.
Disc’s surface and closed to discs events were non-significantly depended on PBL type.
Conclusions:
Results further develop our conception of communicative bodies for cases of Ascomycetes
communities similar to “œYeast like fungi – Filamentous fungi” heterofungal biofilms. PBL may serve
as regulators of earlier mycobiota biofilm forming as well as destructors of Candida based biofilms
involving later Aspergillus or Penicillium. Anti-candidosis agents and factors may be effective
against aspergillosis. PBL-initiated primary and secondary contents of Lacunas can serve as regions
of directed, controlled, diagnostic, prognostic and constructed antimicrobial events, natural locally
different sources of synergistic suitable panels and mixtures of antimicrobials depending on mosaics
of surrounding discs contributors. “œLacunas – Biofilms” can serve as important informative model of
functioning relatively pathogenic fungal communities in the presence of constructed synbiotic noncellular
ingredients and alternatives. New antifungal strategies (for example, against aspergillosis)
are proposed, and new mechanisms of antimicrobial actions (for example, antimycotic ones) of
symbiotic biotope compartment in human are predicted.

Abstract Number: 34

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

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