LAE1 regulates conidiation in Trichoderma atroviride

Ref ID: 18465

Author:

Razieh Karimi Aghcheh,
Irina S. Druzhinina, Christian P. Kubicek

Author address:

Microbiology Group, Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna
University of Technology, 1060 Vienna, Austria  
TU Wien, Institute of Chemical Engineering, Division Applied
Biochemistry and Gene Technol

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Trichoderma atroviride (teleomorph Hypocrea atroviridis) exhibits a synchronized manner of sporulation in
response to light stimulus, and thus serves as a model organism for photomorphogenetic investigations. However,
conidiation of Trichoderma atroviride strongly depends on carbon source. Here we show the involvement of the
LAE1 protein in light sensing by T. atroviride and regulation of its development. Phylogenetic analysis shows that
LAE1 from T. atroviride is orthologous to LAE1 from T. reesei (teleomorph Hypocrea jecorina) and they both are
orthologous to LaeA from Aspergillus nidulans (teleomorph Emericella nidulans). In the later fungus this protein is
the master regulator of secondary metabolite production. A loss of function of lae1 in T. atroviride dramatically
reduces sporulation in general and weakens the induction of conidia formation by light. We compare
photostimulation of conidiation of lae1 mutants of T. atroviride P1 (lae1 knockout and lae1OE respectively) and
the corresponding lae1 mutants of T. reesei by using a broad set of carbon sources in an optimized BIOLOG
Phenotype MicroArray. We also show that the two blue light receptor proteins BLR8208;1 and BLR8208;2 interact with LAE1
in the developmental regulation of T. atroviride. Last not least, the mechanical injury of mycelia, which usually
triggers the conidiation in T. atroviride, was not functional in lae1 knockout mutant as compared to parent and
lae1OE strains. These data suggest that developmental regulation is a main target of LAE1 function in T. atroviride.

Abstract Number: PR1.49

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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