Isolation and Molecular Identification of Aspergillus spp  from ICU, Indoor and Outdoor Environments of Hospitals , Northern Iran

Ref ID: 19527

Author:

A Nasrollahi Omran1*, G Mahmoudi2, MT Hedayati3, A Nazemi4, J Hashemi5, A Karami2

Author address:

1Department of Medical Mycology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
2M.S of Microbiology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
3Department of Medical Mycology, Mazandaran University of Medical Sciences,

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Aspergillus spp is able to germinate and grow over a wide range of temperatures and pH conditions,
increasing its ability to adapt to adverse environments. While exposure to Aspergillus spores is
universal, many causal arguments for hospital acquisition of IA can be put forward.
Methods:
From 500 plates of SDA was placed the ground in inside and outside of the ICU of Mazandaran
hospitals, then, we identified the approximates according to macroscopic and microscopic
morphology. DNA sequencing of the ITS1 and ITS4 regions and also differentiation of species
were conducted by the method High Resolution Melting Real-Time PCR. Species identification was
based on sequence similarity using BLAST searches. Sequences were compared to those derived
from type strains deposited in the Gen Bank data base to identify isolates to the species level.
Results:
In total, 350 Apergillus strains were obtainedfrom ICU, indoor and outdoor environments of the
hospitals in Mazandaran province. 15% of the colonies were Aspergillus fumigatus, 35% Aspergillus
niger and 50% were Aspergillus flavus colonies. In this study, with molecular method there was
100% homology between the Aspergillus isolates of indoor and outdoor the ICU, also we recorded
some isolates as a new strains in Gene Bank.
Conclusion:
The melting real-time PCR is a rapid ,can serve as an accurate and suitable method with a specificity
of 100% for the identification of Aspergillus.
Keywords:
Real-Time PCR, Aspergillus, ICU, HRM, ITS

Abstract Number: 54

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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