Involvement of saga complex components in transcriptional regulation in aspergillus nidulans

Ref ID: 18502

Author:

Paraskevi Georgakopoulos,
Robin Lockington,
Joan Kelly

Author address:

The University Of Adelaide  
University Of South Australia  Â 

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The SAGA complex is highly conserved from yeast to humans.   in yeast it is invloved in the regulation of highly
regulated genes that respond to environmental stresses, such as metabolic starvation, DNA damage and heat.
Bioninformatic and proteomic analyses have shown that the components of the SAGA complex are also present in
Aspergillus nidulans.
In A. nidulans, acetate is a repressing carbon source that leads to similar levels of CreA mediate repression as
glucose.  acdX was identified in a mutation screen in A. nidulans to identify genes invloved in acetate repression
but not in glucose repression.  The conservation of the amino acid sequence of AcdX of A. nidulans and Spt8 of
Saccharomyces cerevisiae suggests that the SAGA complex may have a role in acetate repression in A. nidulans,
since Spt8 is a component of the SAGA complex.
CreA has been shown to repress the expression of the alc regulon, which is required for the ethanol utilization
pathway, and two mechanisms have been identified: (i) by the direct repression of the alcR gene, which results in
almost no expression of the alcA and aldA genes or (ii) by the mechanism in which CreA represses both the alcR
gene and the alcA gene indepenently of one another.
We report results of experiments undertaken to confirm whether AcdX and/or SptC, which is the A. nidulans
homologue of the S. cerevisiae SAGA complex component Spt3, are invlolved in the transcriptional regulation of
the alc regulon in glucose or acetate repressing conditions.

Abstract Number: PR7.20

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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