Involvement of FarA transcription factor in fatty acid utilization in Aspergillus oryzae

Ref ID: 18337

Author:

Sharon Marie Garrido , Noriyuki Kitamoto , Akira
1 2
Watanabe , Takahiro Shintani , and Katsuya Gomi

Author address:

Graduate School of Agricultural Science, Tohoku University, Japan; Food Research Center, Aichi
Industrial Technology Institute, Japan.

Full conference title:

Asperfest 8

Abstract:

This zinc finger transcriptional factor FarA in Aspergillus nidulans up-regulates genes required for growth on fatty acids. FarA is widely conserved in
fungi and binds to 5 -CCTCGG-3¹ core sequences in the promoters of their target genes (Hynes et al, 2006). Ctf1 and Por1, orthologs of FarA of A.
¹
nidulans are required for growth on fatty acids in Candida albicans and also for the essential transcriptional activation of genes involved in beta-oxidation
and peroxisomal biogenesis in Yarrowia lipolytica, respectively (Ramirez and Lorenz, 2009; Poopanitpan et al, 2010). FarA transcriptional factor is also
found in the Aspergillus oryzae which has 83% homology of all the amino acid sequences and 97.5% homology of Zn2Cys6 motifs with the A. nidulans.
In this study, farA disruptant in A. oryzae was characterized and expression levels of genes for fatty acid metabolism and peroxisomal biogenesis and
functions were examined. Interestingly, A. oryzae farA disruptants showed indistinguishable growth in fatty acid sources compared to the wild-type,
inconsistent with the growth phenotype of the A. nidulans counterpart. In contrast, expressions of some genes for fatty acid metabolism, such as acyl-CoA
oxidase and acyl-CoA dehydrogenase genes, were significantly reduced in the farA disruptants. These contradicting results suggested that FarA acts not
only the primary transcriptional activator for fatty acid utilization in A. oryzae and another transcriptional factor(s) may regulate other fatty acid metabolic
genes which can be accounted to the differences on the number of genes between these two Aspergilli.

Abstract Number: 15)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster